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Points to consider for ethics committees in human gene therapy trials

Total protein of the cells was extracted and reduced in the presence of 100 mmol/L DTT for 5 min on the boiling water bath, and subsequently alkylated with 50 mol/L iodoacetamide for 30 min at room temperature in the dark

Evidence supports that IGF-1 is more critical to postnatal longitudinal bone growth compared to IGF-2 because the postnatal phenotype of the IGF-1 null is more severe than Igf2 knockouts (see Table 1) [36,125,148,149]

doi: 10.1242/dev.01702

In clinical studies, berberine has been tested in various cancers, including lung cancer (NCT03486496), colon cancer (NCT03281096), and gastric cancer (NCT03609892)

However, a retrospective study from 2001, using 100 mg zinc per day (as sulphate), found only minor benefits but significant side-effects, with patients on zinc having 7.8 times the risk of getting an infection requiring antibiotics and 12.5 times more likely to experience nausea/vomiting