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After treatment, H2DCFDA or PBSF were added to each well at a final concentration of 10 mol L 1 , kept in the dark at 37 C for 30 min

The first is eumelanin, which is responsible for dark brown and black pigments

qPCR analysis was performed utilizing TB Green Premix EX Taq II (Takara Biotechnology Co., Ltd.) in a GENTIER 96 qPCR instrument (Tianlong)

Science 347 , 1260419 (2015)

Unlike others, the absorption of vitamin B12 depends on a protein called intrinsic factor, which is produced in the stomach

TWX is expected to maintain the function of several organs and tissues including the vocal fold, although the specific mechanism of how TWX works for the vocal fold is not known, and future research is warranted to resolve this aspect