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These contrasting effects may be due to specific BCAAs, since the adverse metabolic effects in obese mice appear to be mediated by isoleucine and valine but not by leucine, whose restriction aggravated hepatic steatosis (213)
In addition, catalase (CAT) enzyme levels were measured following the procedures of Aebi (1984), and reduced glutathione (GSH) estimations were made via the protocol described by Beutler et al

The conversion of superoxide anions into hydrogen peroxide, before subsequently metabolized into water by catalase and glutathione (GSH) peroxidase, is facilitated by the metalloprotein superoxide dismutase (SOD)