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It's a mechanical problem

Assessment of DRG explant survival with live Calcein-AM staining DRG explants were treated with 1 g/ml Calcein-AM (AAT Bioquest) in neurobasal media for 1 h at 37C then switched to clear HBSS-based complete media supplemented with HEPES to maintain physiological pH

Reconstituting excessive amounts creates more reconstituted solution than can be used within the stable window

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The decrease in fluorescence (exc =340 nm

Taking smaller-than-standard amounts of a wide range of drugs may help with depression, anxiety, autoimmune disorders, and more