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Subsequently, the expression level of the target gene was determined using a SYBR Green qPCR amplification kit, with ACT1 as the internal reference gene [26]

Restoring gut barrier integrity addresses one of the root drivers of autoimmune inflammation, not just the symptoms

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Researchers must demand evidence-based assurances that storage recommendations are practical and founded on robust scientific principles

It is seldom necessary for clinicians to know and specify what the reference fluid is when levels are ordered