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Description
Then 3 mL culture medium made of Dulbeccos modified Eagles medium with 10% FBS, 100 U/mL penicillin, and 100 mg/mL streptomycin was added to each well and maintained at 37 C in a humidified atmosphere of 95% air and 5% CO 2
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In 1998, he starred as what is arguably his most iconic role, Jeffrey "The Dude" Lebowski, in the Coen brothers' noir comedy The Big Lebowski
