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GST tagged fusion proteins bind to glutathione agarose and, after washing, are eluted with elution buffer with excess glutathione Protein refolding Reducing agents are common additions to protein purification buffers because they help keep many proteins from forming unnatural disulfide bonds and aggregating

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Laborgeprfte Reinheit.

Conclusions Innovative antioxidant-related gene signature and nomogram performed well in assessing GC prognoses
