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Hydroxocobalamin 1mg is the standard UK formulation, typically administered every two to three months for maintenance therapy Self-administration requires GP prescription, competency training from practice nurses, and demonstration of proper injection technique Common injection sites include the thigh (vastus lateralis) for both intramuscular and subcutaneous administration with site rotation essential Serious side effects are rare but anaphylaxis requires immediate 999 call

Chemiluminescent signals were developed with Luminata Forte HRP substrate (Merck Millipore) and observed using an ImageQuant LAS 350 machine (GE Healthcare)

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