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Avoid freeze-thaw cycle to prevent protein degradation

Immunohistochemistry (IHC) and immunofluorescence (IF) assays of NGF confirmed that the pancreatic tumours exhibited significantly higher NGF immunoreactivity compared to normal pancreas (Supplementary Fig

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This cascade not only elevates lipid peroxide levels but also intensifies iron accumulation in myocardial cells, exacerbating ferroptosis and contributing to myocardial cell death and fibrosis (30)