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Key finding: AOD 9604 (0.1-10 mcg/kg) promoted cartilage repair with 52% defect filling versus 8% in controls

Users often alternate 812 week cycles of 5-Amino-1MQ with other NAD+ precursors (NMN, NR) or mitochondrial-targeted peptides like MK 677 to prevent metabolic adaptation

After 72 hours of treatment, CYP3A4 activity was assessed using the P450-Glo CYP3A4 (Luciferin-PFBE) Cell-Based/Biochemical Assay (Promega) according to manufacturer instructions

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