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2.11 Separation and extraction of nuclear and cytoplasmic proteins In this experiment, nuclear and cytoplasmic fractionation was performed using the Nuclear and Cytoplasmic Protein Extraction Kit (Beyotime, P0027) following the manufacturers instructions

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Pathway enrichment analysis via KEGG, using differentially expressed proteins in each phase, identified significant metabolic pathway enrichment throughout development (Fig

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This would indicate that the length of the spacer in the case of recognition of HER2 antigen on MB cell lines is a key point to consider