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A larger number of genes were changed after TNF- stimulation compared to the basal level (2,637 vs

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10.1016/j.cell.2012.03.042 Cell 20 Do VanB.GouelF.JonneauxA.TimmermanK.GeleP.PetraultM.et al (2016)

Expi293 cells (ThermoFisher, A14527) were maintained in Expi293 medium (ThermoFisher) supplemented with 10% HI-FCS, 25 g/mL streptomycin and 25 U/mL penicillin (ThermoFisher)

The internalization of CTR1 is clathrin- and dynamin-dependent and occurs through vesicles that contain Rab5 and EEA1