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Melatonin (N-acetyl-5-methoxy-tryptamine), a pleiotropic and multitasking molecule, is secreted from the pineal gland during the night under normal light-dark conditions

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One of the papers that laid the foundation stones of the pathway was Foyer and Halliwell (Planta 133:2125, 1976)

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Feature GST Pull-Down Immunoprecipitation Binding Moiety GST Tag Antibody (Protein A/G/L) Beads Glutathione Agarose Beads Protein A/G/L Agarose Elution Harshness Mild Harsh Specificity High Varies Experimental Requirement Cloning with GST Tag Antibody availability How to do a GST pull-down