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The homogenized tissue was then incubated at 80 C for 15 min and then centrifuged at 18,000 g at 4 C for 5 min

Eur J Biochem 234:1623

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The stimulation of one or multiple of these PKC isoforms results in various biological processes, including modifications in cell proliferation and differentiation, ion transport across membranes, glucose and lipid metabolism, smooth muscle contraction, and regulation of gene expression [50]