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Severa, M., Zhang, J., Giacomini, E., Rizzo, F., Etna, M

Membranes were blocked with 5% skim milk in PBST (10 mM Na 2 HPO 4 /NaH 2 PO 4 pH 7.5, 0.9% NaCl, and 0.1% Tween 20), incubated with primary antibodies for 1 h at room temperature, washed three times in PBST, and incubated with horseradish peroxidase (HRP)-conjugated secondary antibodies for 1 h at room temperature

kynurenic acid

As the resulting mRNAs are translated, EJCs are displaced by the ribosome and, once translation terminates, the Fe-S cluster protein, ABCE1, is recruited to release the ribosome from the mRNA [86] (Figure 1)

These mechanisms indicate that MGF might employ a multifaceted strategy to enhance cell proliferation, which likely involves altering cell cycle progression and triggering particular molecular pathways crucial for cellular repair and regeneration
