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A total of 48 h after transfection, cells were collected and RNA was extracted using the RNeasy Mini Kit (Qiagen, 74106), according to the manufacturers instructions

Though there is evidence to suggest that people who suffer from Alzheimers disease and dementia are often deficient in this vitamin, studies have found that taking supplements or increasing dietary vitamin B12 does not improve memory function in those living with the disease, according to the Mayo Clinic

Cell Metab (2021) 33(6):120520.e5

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When deciding which solubility tag to use, it will be worth thinking carefully about the characteristics of the protein you are purifying, and which downstream applications you have in mind

Chen Y, Kantarjian H, Estrov Z, Faderl S, Ravandi F, Rey K, Cortes J, Borthakur G