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This was accomplished by down-regulating a crucial Foc fungal gene, and it was verified by executing 6- week-long bioassays in a greenhouse
Magnetofection PEI-MNPs, T0-CRISPR/Cas9-PEI-MNPs, and T3-CRISPR/Cas9-PEI-MNPs (2 g DNA/well) were incubated with cultured cells in serum free media for 30 min followed by addition of 10% FBS
10.1038/234466a0 72 LiuY
These findings highlight multiple therapeutic access points within the NRF2 regulatory network, including transcriptional repression, interference with upstream regulators such as KEAP1, and targeting of downstream effectors such as SLC7A11 or CYP4F11 [39]
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