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S13A and Fig

The pellets were washed three times with washing buffer A (50 mM Tris pH 8.0, 0.1 M NaCl, 1 mM DTT, 0.5% Triton X-100) and once with buffer B (50 mM Tris pH 8.0, 2 M NaCl, 1 mM DTT, and 1 M urea)

Among the factors activating the pathway regulated by Nrf2 is NADPH oxidase-4 (NOX4) [41]

Additionally, germination is only significantly affected under extreme heat, showing low sensitivity at moderate stress levels

Histological scores were determined in a blinded manner by two independent investigators based on the sum of the epithelium and infiltration scores