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Next, the inhibitors were injected at three different concentrations (depending on their K D determined earlier by independent equilibrium measurements) over the CAP sensor chip loaded with wild-type JNK1 and k 3 and k 4 values were determined by fitting the kinetic binding curves using the COPASI biochemical modeling software based on a 2-step reversible binding scheme, where k 1 and k 2 values were fixed 54

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Comparison of microalgal biomass profiles as novel functional ingredient for food products