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Additionally, irisin treatment inhibited the p-AMPK/AMPK signaling pathway while simultaneously activating the p-AKT/AKT and p-mTOR/mTOR signaling pathways (Fig
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[DOI] [PMC free article] [PubMed] [Google Scholar] 27.Meissner F, Molawi K, Zychlinsky A

Hao J, Meng L-Q, Xu P-C, Chen M, Zhao M-H

Chromatographic analysis of the GSH-OPA abduct was performed using reverse-phase chromatography on Agilent 1200 HPLC (Agilent Technologies, Stockport, UK) with G1311a Quaternary pump, G1367a Autosampler (5 L injection), G1316a Column Compartment (37 C) G1321B Fluorescent light detector, fitted with Phenomenex Gemini C18 100X2 mm and an additional guard column (Phenomenex, Macclesfield, UK) and a stepwise solvent gradient of 25 mM NaH 2 PO 4 (pH 6.0) and acetonitrile as the mobile phase

However, the precise mechanisms by which mitochondrial superoxide is released into the cytoplasm or at a membrane to induce lipid peroxidation remain unclear 31,32
