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Three-dimensional analysis of the structure and composition of CA3 branched dendritic spines and their synaptic relationships with mossy fiber boutons in the rat hippocampus

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20 mM Tris-HCl pH 7.4 and 1 mM EDTA) supplemented with 1 cOmplete mini EDTA-free protease inhibitor cocktail (Sigma-Aldrich), incubated on ice for 10 min, lysed using 20 passes through a syringe affixed to a 23G needle and centrifuged at 1,000 g for 10 min

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Kuroda J, Kimura S, Strasser A, Andreeff M , O'Reilly LA, Ashihara E, Kamitsuji Y, Yokota A, Kawata E, Takeuchi M, Tanaka R, Tabe Y, Taniwaki M, Maekawa T

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