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The liver tissues were digested with 5% KOH at 37 C overnight and washed twice with PBS

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and 21740002 to Z.X.) and by the National Key R&D Program of China (2017YFD0200501 to Z.X.)

Trichoplusia ni cell pellets were suspended in 30 mM HEPES (pH 7.4), 50 mM NaCl, 5 mM CaCl 2 , 2 mM MgCl 2 supplemented with protease inhibitors (REF118361 45001, Roche) and benzonase (Merk Millipore)

In blood, it can be found in non-glycosylated and glycosylated forms, with the latter inactive in blood [12,13]

The MRI findings were unremarkable in all the other patients