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[21] After the solution is deposited into muscle, cholecalciferol diffuses into the circulation bound to vitamin-D-binding protein and undergoes 25-hydroxylation in hepatocytes, yielding 25-hydroxyvitamin D, the principal circulating biomarker of status

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In general: If you follow a vegan, vegetarian, or low-meat diet and are not supplementing with vitamin B12 you might be at risk for deficiency
