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subtilis ISW1214 by transformation, and the transformants were cultured in Luria-Bertani broth containing 50 g ml 1 ampicillin at 37 C

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However, during high-fat diet (HFD) feeding, obesity and insulin resistance, the accumulation of medium-chain and long-chain acylcarnitines due to incomplete -oxidation may negatively regulate the expression of nuclear-encoded mitochondrial genes, further reducing mitochondrial number and function and creating a vicious metabolic cycle [33]

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The body can make carnitine from lysine and methionine which are two of the essential amino acids, however this process only equates to small amounts