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TEER Measurement To assess barrier integrity and functionality of the hIEC and KORTEC models, TEER was measured using an epithelial volt/ohm meter (EVOM2, World Precision Instruments, Sarasota, FL, USA) according to the manufacturers instructions

The complex was eluted using the buffer comprised of 20 mM HEPES pH 7.5, 100 mM NaCl, 0.01% MNG, 0.001% CHS, 1 M ghrelin or ibutamoren, 200 g/ml Flag peptide

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