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Med Res Rev 35(6):11271155

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In vitro ASK1 activity assay HeLa cells were stimulated with TNF- (20 ng/ml) plus cycloheximide (10 g/ml) for 2 h, rinsed once with ice-cold PBS, and lysed in lysis buffer

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The method of embodiment 154, wherein the composition comprises between about 0.01% to about 20% by weight of the cholesterol, about 0.01% to about 15% by weight of the cholesterol, about 0.01% to about 10% by weight of the cholesterol, about 0.01% to about 7% by weight of the cholesterol, about 0.01% to about 5% by weight of the cholesterol, about 0.01% to about 3% by weight of the cholesterol, about 0.01% to about 2% by weight of the cholesterol, or about 0.01% to about 1% by weight of the cholesterol