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TGF-1/2/3 mRNA levels do not allow the prediction of the respective protein levels as it was previously shown 39

In this context, we recognize the evolving landscape of in vitro liver models and intend to explore PHH spheroid models and co-culture spheroid models, which incorporate non-parenchymal cells, in future studies to further assess their functionality and to provide a comprehensive comparison with our iHLO model, thereby continuously advancing the field

For this purpose, the shapefitting algorithm described in our previous work was used [33]

The innate immune system not only serves as the first line of defense but also shapes adaptive immune responses through cytokine and chemokine secretion, co-stimulatory signals, and direct immune cell interactions ( Figure 3 )

Thus, we can speculate that there may be two metabolic pathways for DEHP in LMB-7

In addition, bulk drug substances must be accompanied by a valid certificate of analysis and must have been manufactured by an establishment registered with FDA