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The amount of glucose infusion depends on various factors such as residual enzyme activity, age and the level of stress due to anxiety or fever

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Toruner, Mujde Akturk, Nuri Cakir, Metin Arslan Gynecological Endocrinology.2015

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Aortic ring assay Abdominal and thoracic aorta of 8-week-old C57BL/6 mice were placed in the wells of an eight-well Nunc Lab-Tek Slide System (Sigma-Aldrich) then overlaid with with 50 L of Matrigel
