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Journal of the American College of Cardiology 1999

Blood 107 , 43004307 (2006)

M.DyckJ

The latter is encoded by UGA and directed by the selenocysteine insertion sequence, SECIS element [28, 29], which serves as a platform for the recruitment of elongation factors of selenocysteine-tRNA [Ser]Sec (Sec-tRNA [Ser] Sec ) translation that decodes the UGA codon for the whole family of selenoproteins

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After that, the mixture was transferred into the column and pre-washed with wash buffer (PBS, contained 0, 10, or 30 mmol/L imidazole, pH = 7.6), followed by elution buffer (PBS, contained 300 mmol/L imidazole, pH = 7.6)