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and the stem cell markers OCT4 (Forward: 5-GAGCCCTGCACCGTCACC-3 and Reverse: 5-TTGATGTCCTGGGACTCCTCC-3), SOX2 (Forward: 5-TACAGCATGTCCTACTCGCAGC-3 and Reverse: 5-GAGGAAGAGGTAACCACAGGGG-3), CXCR4 (Forward: 5-ACTACACCGAGGAAATGGGCT-3 and Reverse: 5-CCCACAATGCCAGTTAAGAAGA-3), and cMET (Forward: 5-AGCGTCAACAGAGGGACCT-3 and Reverse: 5-GCAGTGAACCTCCGACTGTATG-3) for 30 cycles using GoTaq qPCR Master Mix (Promega, Madison, WI, USA)
muciniphila was significantly decreased in the intestines of both mice and patients after radiotherapy, and A
The modified protocol demonstrates superior performance characteristics compared to conventional GPx assays, including elimination of interference effects, enhanced accuracy and precision, and improved reproducibility across diverse sample matrices

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