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Functional annotation highlighted genes potentially involved in AFB1 detoxification, including predicted cytochrome P450, epoxide hydrolase (LAP2), glutathione-S-transferase (URE2), and the ABC transporter (YCF1), suggesting pathways for AFB1 activation, glutathione conjugation, and vacuolar sequestration

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Functional enrichment analysis (FEA) supported increased lipoprotein binding and macrophage activation in mens valves, confirmed by increased CD74+cell infiltration (immunohistochemistry)