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The unpaired t-tests were either done assuming equal variance (if p-values for F-tests for equal variance were greater than 0.05), or assuming unequal variance (if F-test p-value results were less than 0.05)

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This may reveal the suggested particular vascular function recovery in glaucoma and retina ischemia, pupil function controlling (counteracted glaucoma-mydriasis, atropine-mydriasis, and NO-agents-miosis) [11,12,13], and healing of severe corneal lesions and recovering corneal transparency (i.e., corneal ulcer, complete denudation of cornea) [14,15]

A deficiency often takes some time to develop, and may not always show symptoms immediately