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In support of this increased NK cytotoxic potential, the expression of the NK cytotoxic effector-associated gene perforin, as determined by flow-cytometric analysis or quantitative real-time PCR analysis in CD56 + NK cells, was also significantly increased in the above IGF-1-induced CD34 + -derived NK cells compared with the cells without IGF-1 (Fig

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PD-1-mediated inhibition of T cell activation: Mechanisms and strategies for cancer combination immunotherapy