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In the hypothalamus, early-life stress resulted in a marked atrophy of astrocytes, aberrant purinergic signaling and limited supply of L-lactate, leading to a pathological activity of orexin neurons driving aberrant behaviors 277

Minami SB, Sha SH, Schacht J

Cells were harvested when the percentage of viable cells as measured in a Casy cell counter (OLS OMNI Life Science) dropped below 88%, usually on day 4 or 5

Sci Adv

During which time I have seen firsthand the importance of prioritizing health and community

The only reason to think about washouts is at very aggressive daily doses, and even then its an enzyme-economy issue rather than a receptor issue
