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When TNF- binds to its receptors TNFR1/2, it activates a TRAF2-mediated JNK-dependent kinase cascade, sequentially activating MKKs, MAPKs, and ultimately p38 MAPK

It plays a natural role in the bodys energy pathways

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Furthermore, the protein content was determined in lysed PBMC to generate a normalization factor, using a bicinchoninic acid assay (BC Assay, Uptima, Interchim, Montluon, France)
