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Using a reagent called crosslinking agent or crosslinker the immobilization process is carried out, which form intramolecular and intermolecular crosslinks with specific groups of amino acids present on the surface of the solubilized enzyme consequently formed crosslinked enzymes [160, 173]

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In one large series, the mean largest basal dimension was 11.1 mm and average thickness was 5.5 mm

On USG, it presents as single or multiple hyperechoic lesions with variable echogenicity due to hemorrhages

[5] The hydrolytic products also go on to modulate the activity of downstream proteins important for cellular signaling