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Clinical data suggests that GIP co-activation with GLP-1 may also reduce nausea rates compared to GLP-1 stimulation alone, which could explain why Retatrutide and Tirzepatide cause less nausea than Semaglutide despite being more potent compounds

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RNA isolation and relative quantitative real-time PCR (qRTPCR) Total RNA was extracted from different cells with a Fastgen2000 RNA isolation system (Fastgen, Shanghai, China) according to the manufacturers protocol

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