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PI3K/Akt inhibitor partly decreases TNF-alpha-induced activation of fibroblast-like synoviocytes in osteoarthritis
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[DOI] [PubMed] [Google Scholar] Carr A, Frei B
Biochemistry 2003
[DOI] [PubMed] [Google Scholar] Freeman B
The samples were washed four times in PBST, solubilized in Laemmli buffer and subjected to Western blot analyses as described above
