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Mouse primary muscle cell culture Cultures were established from mixed hindlimb muscle of 1 month old Cpt1b m/ and Cpt1b fl/fl littermates 24

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Previous work has shown that the abundance of Lactobacillus reuteri (normalized to a control group) decreases during the onset of obesity and remains suppressed even after dieting in mice 99 , which parallels our observations that vehicle-treated diet-switched mice had lower levels of Lactobacillus than the 5A-1MQ-treated mice, the latter of which also demonstrated greater weight loss