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Following various treatments, the MTT reagent was added and incubated for 4 h, after which the formazan crystals were solubilized and absorbance was measured at 570 nm using a microplate reader [36]

Following induction of anesthesia, animals were placed in the prone position for dorsal trichotomy

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4,5 A key advantage in the study of APAP toxicity is that its human pathophysiology can be effectively reproduced in a mouse model in vivo and in primary mouse hepatocytes