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Dissociated tumor cells were strained through a 70 m filter and washed with FACS buffer containing HBSS (Thermofisher, Cat # 14175095), 0.5 mM EDTA (Sigma-Aldrich, Cat # E7889-100 ML), and 2.5% FBS (Gibco, Cat # 16000044)

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High prevalence of insulin resistance is suggested in patients with PD ranging from 50 to 80% and type 2 diabetes is associated with an increased risk of PD (151)

Symptoms occurring with this condition include knee instability and may be related to damage to other joint structures such as the meniscus or the tibial collateral ligament