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Finally, the TS2/16 scFv was purified on size-exclusion chromatography with Superdex 75 Increase 10/300 GL column (Cytivia) on the KTA pure chromatography system (Cytivia) in 1 HBS running buffer (20 mM HEPES pH 7.3, 300 mM NaCl)

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## mean.ER : mean encounter rate across simulation
