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This comprehensive experimental design ensures controlled conditions, adequate sample size, and systematic data collection, allowing for the evaluation of DSIPs efficacy in ameliorating neurotransmitter imbalance and enhancing sleep in PCPA-induced insomnia mouse models

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Parkinson's disease: Glutathione depletion in the substantia nigra is one of the earliest biochemical changes in Parkinson's disease, occurring before dopaminergic neuron loss becomes apparent [49]