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The cells were incubated at 37 C under a 5% CO 2 /95% air atmosphere

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When we included LiPA results for those samples in which sequencing was not done, overall agreement at the subtype level (with either LiPA or sequencing) even slightly increased to 98.6% (140/142), as the agreement at HCV-1 subtype calling between the GT Plus and the LiPA assays was 100% (all being 1b except one 1a case)