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Cells were treated with 5 or 10 mol/ml ASA (dissolved in 1 M Tris-HCl (pH 7.6) to a stock solution of 1 M and adjusted to pH 7.45 with 4 N HCl) for 48 hours

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Post-traumatic epilepsy (PTE) develops in as many as 50% of TBI patients, and TBI and chronic epilepsy brain tissue both display elevated markers of oxidative stress in addition to cytokines and neurodegeneration (Vezzani et al., 2008

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The molar ratio was 1.5 biotin per hHGF as determined using a Biotin Quantitation Kit (Pierce)

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