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A related class of nutrient, which has not been demonstrated as necessary or essential for life yet provides health benefits when added at levels greater than a normal diet generally provides, has come to be known as nutraceuticals, a coinage based on an amalgamation of nutrition and pharmaceutical (11)

Acknowledgments The authors would like to thank the National Council for Scientific and Technological Development (CNPq) (Grant: 448905/2014-0 to A.A.S.R.)

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Cancer 10 (19), 47194730

Plasmid constructions and reporter activity assay The 1200-bp DNA fragment containing the CBS promoter region from 1000 to +200 bp was amplified from SKOV3 Era-R cell genomic DNA by PCR with the following primers: CBS- Kpn I forward, 5-GGTACCGACATTTAATTCTAATTCACGTCTC-3 and CBS- Bgl II reverse, 5-AGATCTGTCCAGAGAGGGGAGCGAGTCTCGG-3

Lin Yuan, Email: [email protected], Email: [email protected]