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Purification of HaloTag Fusion Proteins HaloTag Protein Purification from Mammalian Cells Cultured mammalian cells offer an environment well suited for producing properly folded and functional mammalian proteins with appropriate post-translational modifications

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DNA amplifications were performed in a Bio-Rad CFX-96 real-time thermocycler (Bio-Rad, USA), running a standard program (95C for 2 min, 40 cycles at 95C for 10 s, 60C for 10 s and 72C for 30 s, followed by a melting curve analysis to determine amplicon specificity using a temperature range from 65 to 95C with increments of 0.5C)

Table 1 Ferroptosis related targets and molecules