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The cell suspension was centrifuged at 500 g for 5 min at 4C and the cell pellet was incubated with ACK lysing buffer (Thermo Fisher Scientific, A1049201) for 5 minutes to lyse the red cells
2'-Hydroxy-4-methoxychalcone inhibits both the NF-B and AP-1 signaling pathways, exerting anti-inflammatory activity
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