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Data are presented as the number of flinching

Add secondary peptides after establishing tolerance and seeing results

An ADVANCE low flow Michrom source (Bruker-Michrom) was used to ionize peptides as they elute from the capillary column in a linear gradient from 100% buffer A (2% acetonitrile in water containing 0.1% formic acid) to 15% buffer A plus 85% buffer B (100% acetonitrile in water containing 0.1% formic acid) over 80 min

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Results for frozen shoulder vary considerably

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