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In the current investigation, in comparison with the control group, NaF administration significantly decreases sperm count, sperm viability, sperm motility, normal sperm morphology and increased abnormal sperm morphology by subjecting the spermatozoa to increased oxidative stress and DNA damage because their plasma membranes contain large quantities of polyunsaturated fatty acids.42 Increased formation of ROS has been correlated with a reduction of sperm motility.43 The role of fluoride toxicity on spermatogenesis could be due to reductions of testosterone levels by NaF and by reducing the testicular zinc levels, thereby impairs angiotensin-converting enzyme (ACE) activity and thus causes inhibition of spermatogenesis.44 The mechanism by which fluoride affects sperm motility has not been clearly elucidated

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